LMI Pathobios

MYCOMOL TRAINING

Molecular diagnosis and characterization of genetic diversity in fungal crop pathogens

Background and justification:

The main goal of this training is to contribute to capacity building on the use of new technologies for diagnosis and characterization of the genetic diversity of fungal biodiversity.

 

Specific goals

  1. To remind some theoretical bases necessary for the implementation of molecular detection and characterization of the genetic diversity of fungal bio-aggressors:  genomes structure, types of genetic labels, theoretical bases of molecular diagnosis, etc.
  2. To put in practice the molecular detection tools and characterization of the diversity of bio-aggressors: DNA extraction, amplification and revelation, sequencing or genotyping
  3. To interpret or tap from the data produced: sequencing products analysis, sequence lining, polymorphism analysis, determining diversity, population structure analysis

Expected outcomes:

At the end of the training, the theoretical knowledge of the interns will be reinforced on the detection tools and the analysis of fungal pathogen population diversity.

The interns would be able to carry out the different technical stages applied during the training.

The interns will have a basic training to interpret and use the results gained.

Organizers:

UNB, INERA, IRD, Patho-Bios International Joint Laboratory

Pedagogical team:

Cirad, INERA, IRD, Patho-Bios International Joint Laboratory

Monday, June 11, 2018

Welcoming guests, presentation on the goals of the training and theoretical reminders in molecular biology

Tuesday, June 12, 2018

Practical manipulations: fungal DNA extraction

Wednesday, June 13, 2018

Practical manipulations: PCR and revelation on agarose gel

Thursday, June 14, 2018

Data analysis (DNA and microsatellite sequences)

Friday, June 15, 2018

Data analysis (DNA and microsatellite sequences) and training report

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